PhenoChip: A single-cell phenomic platform for high-throughput photophysiological analyses of microalgae
Lars Behrendt, M. Mehdi Salek, Erik Trampe, Vicente I. Fernandez, Kang Soo Lee, Michael Kühl, Roman Stocker
Abstract
to thermal and chemical treatments and monitor single-cell photophysiology via chlorophyll fluorometry. This revealed strain-specific thermal sensitivity thresholds and distinct pH optima for photosynthetic performance, and permitted the identification of single cells with elevated resilience toward rising temperature. Optical expulsion technology was used to collect single cells from PhenoChip, and their propagation revealed indications of transgenerational preservation of photosynthetic phenotypes. PhenoChip represents a versatile platform for the phenotyping of photosynthetic unicells relevant to biotechnology, ecotoxicology, and assisted evolution.