Principles and Applications of Single Particle Tracking in Cell Research
Zhao Wang, Xuejing Wang, Ying Zhang, Weili Xu, Xiaojun Han
Abstract
It is a tough challenge for many decades to decipher the complex relationships between cell behaviors and cellular physical properties. Single particle tracking (SPT) with high spatial and temporal resolution has been applied extensively in cell research to understand physicochemical properties of cells and their bio-functions by tracking endogenous or exogenous probes. This review describes the fundamental principles of SPT as well as its applications in intracellular mechanics, membrane dynamics, organelles distribution, and processes of internalization and transport. Finally, challenges and future directions of SPT are also discussed.
Topics & Concepts
InternalizationTracking (education)NanotechnologyDECIPHEROrganelleIntracellularParticle (ecology)CellBiological systemComputer scienceMaterials scienceChemistryBiologyCell biologyBioinformaticsBiochemistryPsychologyEcologyPedagogyAdvanced Fluorescence Microscopy TechniquesRNA Interference and Gene DeliveryNanoparticle-Based Drug Delivery