Targeted base editing in the plastid genome of Arabidopsis thaliana
Issei Nakazato, Miki Okuno, Hiroshi Yamamoto, Yoshiko Tamura, Takehiko Itoh, Toshiharu Shikanai, Hideki Takanashi, Nobuhiro Tsutsumi, Shin‐ichi Arimura
Abstract
Abstract Bacterial cytidine deaminase fused to the DNA binding domains of transcription activator-like effector nucleases was recently reported to transiently substitute a targeted C to a T in mitochondrial DNA of mammalian cultured cells 1 . We applied this system to targeted base editing in the Arabidopsis thaliana plastid genome. The targeted Cs were homoplasmically substituted to Ts in some plantlets of the T 1 generation and the mutations were inherited by their offspring independently of their nuclear-introduced vectors.
Topics & Concepts
Arabidopsis thalianaPlastidBiologyGenomeGenome editingComputational biologyArabidopsisGeneticsGeneChloroplastMutantPhotosynthetic Processes and MechanismsCRISPR and Genetic EngineeringPhotoreceptor and optogenetics research