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IL-13Rα2 humanized scFv-based CAR-T cells exhibit therapeutic activity against glioblastoma

Chang Xu, Yue Bai, Zhijing An, Yi Hu, Can Zhang, Xiaosong Zhong

2022Molecular Therapy — Oncolytics21 citationsDOIOpen Access PDF

Abstract

and in two xenograft mouse models. However, T cells transduced with humanized CAR have an increased expansion and reduced cytokines, including interleukin-6 and interferon-γ. The top expressed genes clustered in leukocyte-mediated cytotoxicity, and T cell migration and immunological synapse formation contributed to the anti-glioblastoma (GBM) activity of the humanized CAR. In conclusion, we successfully generated a humanized third-generation CAR-targeting IL-13Rα2 and confirmed its anti-GBM efficacy, which provide a candidate method for clinical GBM treatment.

Topics & Concepts

GlioblastomaCancer researchHumanized antibodyMedicineAntibodyImmunologyMonoclonal antibodyCAR-T cell therapy researchViral Infectious Diseases and Gene Expression in InsectsNanowire Synthesis and Applications