Establishment of ultrasound-responsive SonoBacteriaBot for targeted drug delivery and controlled release
Meng Du, Ting Wang, Renjie Feng, Penghui Zeng, Zhiyi Chen
Abstract
Bacteria-driven biohybrid microbots have shown great potential in ca n cer treatment. However, how precisely controlling drug release at the tumor site is still an issue. To overcome the limitation of this system, we proposed the ultrasound-responsive SonoBacteriaBot (DOX-PFP-PLGA@ EcM ). Doxorubicin (DOX) and perfluoro-n-pentane (PFP) were encapsulated in polylactic acid-glycolic acid (PLGA) to form ultrasound-responsive DOX-PFP-PLGA nanodroplets. Then, DOX-PFP-PLGA@ EcM is created by DOX-PFP-PLGA amide-bonded to the surface of E. coli MG1655 ( EcM ). The DOX-PFP-PLGA@ EcM was proved to have the characteristics of high tumor-targeting efficiency, controlled drug release capability, and ultrasound imaging. Based on the acoustic phase change function of nanodroplets, DOX-PFP-PLGA@ EcM enhance the signal of US imaging after ultrasound irradiation. Meanwhile, the DOX loaded into DOX-PFP-PLGA@ EcM can be released. After being intravenously injected, DOX-PFP-PLGA@ EcM can efficiently accumulate in tumors without causing harm to critical organs. In conclusion, the SonoBacteriaBot has significant benefits in real-time monitoring and controlled drug release, which has significant potential applications for therapeutic drug delivery in clinical settings.