Translation-coupled RNA replication and parasitic replicators in membrane-free compartments
Ryo Mizuuchi, Norikazu Ichihashi
Abstract
We report RNA self-replication through the translation of its encoded protein within membrane-free compartments generated by liquid-liquid phase separation. The aqueous droplets support RNA self-replication by concentrating a genomic RNA and translation proteins, facilitating the uptake of small substrates, and preventing the replication of parasitic RNAs through compartmentalization.
Topics & Concepts
Translation (biology)Replication (statistics)RNACell biologyChemistryEukaryotic translationBiophysicsBiologyComputational biologyVirologyBiochemistryMessenger RNAGeneRNA and protein synthesis mechanismsRNA Research and SplicingRNA modifications and cancer