Ultrasensitive Photoelectrochemical Biosensor for microRNA-155 Based on Energy Transfer between Au Nanocages and Red Emission Carbon Dot-Assembled Nanosheets Coupled with the Duplex-Specific Nuclease Enzyme-Assisted Target Recycling Strategy
Yang Jiao, Fang Luo, Jian Wang, Bin Qiu, Jie Shen, Lin Zhang, Zhenyu Lin
Abstract
-modified FTO serving as the working electrode, and a quenched photocurrent was detected. In the presence of the target, the disintegration of the nanocomposite was induced through target hybridization and DNA hydrolyzation, leading to the separation of AuNCs and R-CDs NS, and the ET disappeared and led to a high photocurrent. With duplex-specific nuclease enzyme-assisted target recycling, the high sensitivity enabled the sensor to monitor the target in cancer cells. The sensor has a low detection limit of 71 aM. The sensing platform has high sensitivity, good selectivity, and reproducibility.
Topics & Concepts
PhotocurrentNanocagesChemistryBiosensorNucleaseElectron transferAbsorption (acoustics)PhotochemistryFluorescencePhotoelectrochemistryNanocompositeNanotechnologyElectrodeOptoelectronicsDNAMaterials scienceElectrochemistryComposite materialBiochemistryCatalysisPhysical chemistryQuantum mechanicsPhysicsAdvanced biosensing and bioanalysis techniquesCarbon and Quantum Dots ApplicationsAdvanced Nanomaterials in Catalysis