Genetic Diversity Analysis of Indian Mustard (Brassica spp.) Germplasm Lines using SSR Molecular Markers
Ravi S. Baghel, Anil Kumar Sharma, Sushma Tiwari, M. K. Tripathi, Niraj Tripathi
Abstract
Molecular categorization of 48 mustard (Brassica spp.) genotypes by employing 20 SSR markers divulged that eight primers viz., BRMS-240, BRMS-324, SR-7223, SR-9222, SR-94102, OI10-C05, SSRNa10-DO9 and SSRNa 10-D11 illustrated good technical resolution and out of these eight seven furnished adequate variations among diverse genotypes. In total 50% polymorphism was detected. Major group restrained 48 genotypes that divided into three major groups, first main group ‘I’ contained 17 genotypes, second chief group ‘II’ hold 24 genotypes and third core group ‘III’ included 7 genotypes. Polymorphic information content (PIC) was estimated for each of the 7 SSR markers. The highest PIC value (0.6851) was documented for the molecular marker OI10-CO 5. It had 8 alleles among the 48 genotypes; whereas, the lowest PIC (0.4038) was examined for the molecular marker SR-7223.