Litcius/Paper detail

Polymer architecture orchestrates the segregation and spatial organization of replicating<i>E. coli</i>chromosomes in slow growth

Debarshi Mitra, Shreerang Pande, A. C. Chatterji

2022Soft Matter17 citationsDOI

Abstract

, 2597-2609. We also show that the adoption of such modified polymer architectures by the daughter DNAs leads to an enhanced propensity of their spatial segregation. Secondly, we match other experimentally reported results, including observation of the cohesion time and the ter-transition. Additionally, the contact map generated from our simulations reproduces the macro-domain like organization as seen in the experimentally obtained Hi-C map. Lastly, we have also proposed a plausible reconciliation of the 'Train Track' and the 'Replication Factory' models which provide conflicting descriptions of the spatial organization of the replication forks. Thus, we reconcile observations from complementary experimental techniques probing bacterial chromosome organization.

Topics & Concepts

Circular bacterial chromosomeChromosomeDNADNA replicationPolymerCell divisionTelomereBiologyGeneticsBiological systemTopology (electrical circuits)BiophysicsPhysicsCellMathematicsGeneCombinatoricsNuclear magnetic resonanceBacterial Genetics and BiotechnologyBacteriophages and microbial interactionsDiffusion and Search Dynamics