Association between Exosomal miRNAs and Coronary Artery Disease by Next-Generation Sequencing
Sheng‐Nan Chang, Jien‐Jiun Chen, Jo‐Hsuan Wu, Yao-Te Chung, Jin-Wun Chen, Chu-Hsuan Chiu, Chia‐Ju Liu, Meng-Tsun Liu, Yi‐Cheng Chang, Chin Li, Jou‐Wei Lin, Juey‐Jen Hwang, Wen-Pin Lien
Abstract
BACKGROUND: Among various bio-informative molecules transferred by exosomes between cells, micro RNAs (miRNAs), which remain remarkably stable even after freeze-and-thaw cycles, are excellent candidates for potential biomarkers for coronary artery disease (CAD). METHODS: Blood samples were collected from the coronary arteries of 214 patients diagnosed with three-vessel CAD and 140 without CAD. After precipitation extraction, the amounts of exosomes were found to decrease with increased age and three-vessel CAD. Next-generation sequencing was performed to further explore the possible relationship between exosomal miRNAs and CAD. RESULTS: Eight exosomal miRNAs showed altered expression associated with CAD. The up-regulated miRNAs in CAD were miRNA-382-3p, miRNA-432-5p, miRNA-200a-3p, and miRNA-3613-3p. The down-regulated miRNAs were miRNA-125a-5p, miRNA-185-5p, miRNA-151a-3p, and miRNA-328-3p. CONCLUSION: We successfully demonstrated particular exosomal miRNAs that may serve as future biomarkers for CAD.