Identification and Spatial Visualization of Dysregulated Bile Acid Metabolism in High-Fat Diet-Fed Mice by Mass Spectral Imaging
Qi Zhang, Zhenhua Wu, Shan-Shan Zhao, Jing Yang, Lei Chen, Xiaoyu Wang, Zhan‐You Wang, Huixin Liu
Abstract
-1-naphthylphthalamic acid (NPA). Our results could clearly determine the zonation patterns and regional difference characteristics of BAs on mouse liver, ileum, and colon tissue sections, and the relative content of BAs based on NPA could also be ascertained. In conclusion, our method promoted the accessibility of spatial localization and quantitative study of BAs on gastrointestinal tissue sections and demonstrated that MALDI-MSI was a valuable tool to investigate and locate several BA molecules in different tissue types leading to a better understanding of the role of BAs behind the gastrointestinal diseases.
Topics & Concepts
Identification (biology)Bile acidMetabolismVisualizationLipid metabolismMass spectrometry imagingChemistryEndocrinologyInternal medicineBiologyBiochemistryMass spectrometryMedicineComputer scienceArtificial intelligenceChromatographyEcologyLiver Disease Diagnosis and TreatmentMetabolomics and Mass Spectrometry StudiesMetabolism and Genetic Disorders