Dietary nitrate increases submaximal SERCA activity and ADP transfer to mitochondria in slow-twitch muscle of female mice
Heather L. Petrick, Stuart Brownell, Bayley Vachon, Henver S. Brunetta, Rachel M. Handy, Luc J. C. van Loon, Coral L. Murrant, Graham P. Holloway
Abstract
We show that nitrate supplementation increased force production during fatigue and increased submaximal SERCA activity. This was also evident regarding the high-energy phosphate transfer from SERCA to mitochondria, as nitrate increased mitochondrial respiration supported by SERCA-derived ADP. Surprisingly, these observations were only apparent in muscle primarily expressing type I (soleus) but not type II fibers (EDL). These findings suggest that alterations in SERCA properties are a possible mechanism in which nitrate increases force during fatiguing contractions.
Topics & Concepts
SERCAMitochondrionInternal medicineNitrateChemistryEndocrinologySoleus muscleHigh-energy phosphateSkeletal muscleBiochemistryBiologyEnergy metabolismATPaseMedicinePhosphocreatineOrganic chemistryEnzymeMuscle Physiology and DisordersMuscle metabolism and nutritionAdipose Tissue and Metabolism