CUT&Tag Data Processing and Analysis Tutorial v1
Ye Zheng, Kami Ahmad, Steven Henikoff
Abstract
This tutorial is designed for processing and analyzing CUT&Tag data following the Benchtop CUT&Tag V.3 protocol. The illustration data used in this tutorial is the profiling of histone modifications in the human lymphoma K562 cell line, but the tutorial is generally applicable to any chromatin protein, including transcription factors, RNA polymerase II, and epitope-tagged proteins. For reproducible analysis, this tutorial is also available on GitHub at https://yezhengstat.github.io/CUTTag_tutorial/.
Topics & Concepts
Computer scienceHistoneComputational biologyTranscription (linguistics)AnnotationProfiling (computer programming)RNA polymerase IIChemistryProgramming languageBiochemistryBiologyGene expressionArtificial intelligenceDNAGenePhilosophyLinguisticsPromoterGenomics and Chromatin DynamicsCancer-related gene regulationRNA modifications and cancer