Predictive Power of Long-Read Whole-Genome Sequencing for Rapid Diagnostics of Multidrug-Resistant Brachyspira hyodysenteriae Strains
Nick Vereecke, Nadine Botteldoorn, C. Brossé, Caroline Bonckaert, Hans Nauwynck, Freddy Haesebrouck, Filip Boyen, Dominiek Maes, Sebastiaan Theuns
Abstract
species using molecular techniques. Nevertheless, no quick diagnostic tools are available for antimicrobial susceptibility testing due to extended growth requirements (7 to 14 days). To enable practitioners to tailor antimicrobial treatment to specific strains, long-read sequencing-based methods are expected to lead to rapid methods in the future. Nevertheless, their potential implementation should be validated extensively. This mainly implies assessing sequencing accuracy and the predictive power of genetic hallmarks in relation to their observed (multi)resistance phenotypes.
Topics & Concepts
BiologyIdentification (biology)Multiple drug resistanceGenomeVirologyComputational biologyMicrobiologyDrug resistanceGeneticsGeneBotanyVeterinary medicine and infectious diseasesMycobacterium research and diagnosisClostridium difficile and Clostridium perfringens research