A minor structure modification serendipitously leads to a highly carbapenemase-specific fluorogenic probe
Jie Wang, Weipan Xu, Shuyuan Xue, Tao Yu, Hexin Xie
Abstract
Reported herein is a fluorogenic probe for the detection of carbapenemase activity. This reagent features carbapenem as an enzyme recognition motif and a carbon-carbon double bond between carbapenem and the fluorophore, exhibiting high specificity to all carbapenemases, including metallo carbapenemases and serine carbapenemases, over other β-lactamases.
Topics & Concepts
CarbapenemSerineChemistryReagentCombinatorial chemistryMicrobiologyBiologyBiochemistryEnzymeAntibioticsOrganic chemistryAntibiotic Resistance in BacteriaPneumocystis jirovecii pneumonia detection and treatmentPharmaceutical and Antibiotic Environmental Impacts