Development of an Efficient C-to-T Base-Editing System and Its Application to Cellulase Transcription Factor Precise Engineering in Thermophilic Fungus <i>Myceliophthora thermophila</i>
Chenyang Zhang, Nan Li, Lang Rao, Jingen Li, Qian Liu, Chaoguang Tian
Abstract
CLR-2 (MtCLR-2) were successfully generated via our base editor Mtevo-CDA1 to elucidate its function. Here, we show that the DNA-binding domain of MtCLR-2 is important for the fungal response to cellulose conditions, while its fungus-specific motif is involved in fungal growth. These findings indicate that our base editor can be an effective tool for elucidating the functions of motifs of target genes in filamentous fungi and for metabolic engineering in the field of synthetic biology.
Topics & Concepts
BiologyGenome editingThermophileCytosineCytosine deaminaseGeneticsCRISPRGeneCellulaseComputational biologyBiochemistryEnzymeBacteriaGenetic enhancementCRISPR and Genetic EngineeringInsect symbiosis and bacterial influencesFungal and yeast genetics research