Potential of cotton sticks-derived biochar to alleviate Aluminium toxicity in cotton

Abstract

Aluminium (Al 3+ ) toxicity is a significant challenge for crops, mostly because of excessive soil acidity resulting from continuous agricultural activity and altered environmental conditions associated with climate change, which liberate metal ions. Biochar, derived from agricultural and other organic waste, can serve as an onsite remediation technique that improves soil quality in regions affected by (heavy) metals. There is a lack of studies in the literature regarding the function of biochar to mitigate negative impacts of aluminium on cotton, so this experiment was conducted to give novel insights to farmers for sustainable cotton productivity. This work aimed to investigate the potential of cotton sticks-derived biochar in mitigating aluminium toxicity in cotton (Var. FH-492). The experiment utilized varying quantities of biochar generated from cotton sticks (0 %, 2 %, 4 %, and 6 % w/w) in conjunction with varied aluminium levels (0, 15, 30, and 45 mg/L), following a completely randomized design (CRD). Findings indicate that AlCl 3 , at all concentrations, lowered the length and dry weight of shoots and roots, tolerance index, Hill reaction, concentrations of chlorophyll a, chlorophyll b, and dissolved proteins, while increasing root and shoot aluminium levels (up to 720.60 and 80.1 times, respectively), the percentage of cell death (59 %), hydrogen peroxide levels (67.14 %), MDA (malondialdehyde) levels (50.92 %), proline, and dissolved sugars. Flavonoids content and total phenolics content, percentage of DPPH free radical scavenging, and ascorbate peroxidase and catalase activity were also increased in cotton leaves with increased aluminium concentration incorporated into growth media. The inclusion of biochar into the substrate of aluminium-stressed cotton plants reduced the bioavailability of aluminium and thereby the aluminium deposition in cotton roots and shoots (by 69.47 % and 78.24 %, respectively). The findings revealed the efficacy of biochar in diminishing aluminium bioavailability and enhancing aluminium fixing in aluminium-contaminated soils. Biochar proved most effective at a dose of 6 % (w/w). • Aluminum toxicity significantly reduced cotton growth, biochemical and physiological parameters • Biochar significantly enhanced cotton biomass level and growth • Biochar decreased aluminum bioavailability, thereby reducing its accumulation in cotton plants • Biochar mitigated aluminum toxicity by antioxidant enzyme activity in cotton

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