New Antifungal and Antifeedant Metabolites from <i>Daldinia Eschscholtzii</i> Cocultured with <i>Colletotrichum Pseudomajus</i>
Jingxin Chen, Xue-Qiong Yang, Jing Sun, Yu-He Li, Ya‐Bin Yang, Zhong‐Tao Ding
Abstract
Abstract The synchronous co‐culture of Daldinia eschscholtzii and Colletotrichum pseudomajus produced one new linear polyketide, eschscholin C ( 1 ), along with three known compounds ( 2 – 4 ). One new acorane sesquiterpene, coldaldrin A ( 5 ), and one new amide derivative, coldaldamide A ( 6 ) as the probe for polyketide intermediate capture, and three known compounds ( 7 – 9 ) were isolated from the sequential co‐culture of D. eschscholtzii with C. pseudomajus . The structures and absolute configurations of 1 , 5 and 6 were established by spectroscopic analysis including 1D, 2D NMR, the calculations of the NMR, and ECD data. Most compounds showed significant antifungal activities against the tea pathogens C. pseudomajus , and Fusarium asiaticum with MICs of 2–8 μ g/mL. Compound 4 also showed antifeedant activity against silkworms with feeding deterrence indices of 79 % at the concentration of 50 μ g/cm 2 .