Human Mat2A Uses an Ordered Kinetic Mechanism and Is Stabilized but Not Regulated by Mat2B
Jonathan Bailey, Holly Douglas, Laura Masino, Luiz Pedro S. de Carvalho, Argyrides Argyrou
Abstract
of 6 ± 1 nM). Finally, we found that while Mat2A is unstable at low concentrations (<100 nM), rapidly losing activity at 37 °C, it retained full activity for at least 2 h when Mat2B was present at the known 2:1 Mat2A/Mat2B stoichiometry.
Topics & Concepts
Methionine AdenosyltransferaseIsothermal titration calorimetryPyrophosphateChemistryAdenosine triphosphateTransmethylationStereochemistryProduct inhibitionKineticsBinding siteMethionineBiophysicsCooperative bindingKinetic energyBiochemistryEnzymeNon-competitive inhibitionBiologyAmino acidQuantum mechanicsPhysicsRNA modifications and cancerEpigenetics and DNA MethylationFolate and B Vitamins Research