Tuning multi-purpose glycerol-based deep eutectic solvents for biocatalytic deacidification of high-acid rice bran oil: Kinetics, mechanism, and inhibition of side glycerolysis
Pengfei Zhou, Weiqi Liu, Yan Zhang, Guang Liu, Zhihao Zhao, Mingliang Zhang, Mingliang Zhang, Zhencheng Wei, Yan Zhang, Xiaojun Tang, Ping Li, Yuanyuan Deng, Mingwei Zhang, Mingwei Zhang, Zheng Guo
Abstract
Developing an effective deacidification process to utilize high-acid rice bran oil (HRBO) for the production of high-quality and value-added edible products remains challenging. In this study, we developed a novel, multi-purpose, and sustainable lipase-deacidification system for HRBO utilization in glycerol-based deep eutectic solvents (DESs), resulting in a 94.5% deacidification efficiency and simultaneously prevented the glycerolysis side reaction. Importantly, the enzymatic kinetics were investigated in comparison with choline chloride/glycerol DESs and the pure glycerol system. In addition, the underlying mechanism of inhibition accompanied by glycerolysis side reaction in the enzymatic-deacidification of HRBO was proved by molecular docking simulations. We believe our findings demonstrate the potential of glycerol-based DESs as a highly efficient, sustainable, and recyclable solution for the deacidification of edible oils.