Erwinia phyllosphaerae sp. nov., a novel bacterium isolated from phyllosphere of pomelo (Citrus maxima)
Mingkai Pan, Guang-Da Feng, Qing Yao, Jiali Li, Chen‐Jian Liu, Honghui Zhu
Abstract
A novel phosphate-solubilizing and 3-indoleacetic acid producing bacterium, designated strain CMYE1 T , was isolated from the phyllosphere of pomelo ( Citrus maxima ) in Meizhou, Guangdong Province, PR China. Cells were Gram-stain-negative, facultative aerobic, non-spore-forming, rod-shaped and motile with peritrichous flagella. It had the highest 16S rRNA gene sequence similarity to Kalamiella piersonii NRRL B-65522 T (99.0 %), followed by Pantoea cypripedii LMG 2657 T (98.1 %), Erwinia iniecta B120 T (97.7 %), Mixta intestinalis 29Y89B T (97.6 %) and other species (<97.6 %). However, phylogenomic analyses clearly showed that strain CMYE1 T should be assigned into the genus Erwinia , and was most closely related to Erwinia oleae LMG 25322 T (96.7 %). Genome comparisons showed that the novel strain shared ≤83.2 % average nucleotide identity and ≤26.5 % digital DNA–DNA hybridization values with closely related strains, respectively. It contained C 16 : 0 , C 17 : 0 cyclo, summed feature 3 (C 16 : 1 ω 7 c and/or C 16 : 1 ω 6 c ) and summed feature 8 (C 18 : 1 ω 7 c and/or C 18 : 1 ω 6 c ) as the major fatty acids. Based on the results of phylogenetic, phenotypic and chemotaxonomic analyses, as well as genome comparisons, strain CMYE1 T belongs to a novel species of the genus Erwinia , for which the name Erwinia phyllosphaerae sp. nov. is proposed with the type strain CMYE1 T (=GDMCC 1.2674 T =JCM 34792 T ).