Mechanism of reducing antibiotic resistance genes by nano-selenium during composting: insight into host microorganisms and a two-component system
Xi Chen, Muqing Zhan, Haochen Shao, Shuangshuang Ma, Li Liu, Zuyong Chen, Fang Chen
Abstract
Nano-selenium (nano-Se) makes up for the low biological activity and high toxicity of inorganic selenium, and is widely used as a novel Se fertilizer in agriculture. This study investigated the effects of different concentrations of nano-Se on Antibiotic resistance genes (ARGs) and host microorganisms during aerobic composting of cow manure. The results showed that high-concentration nano-Se had excellent effects on ARG removal, effectively inhibiting the dominant ARG subtypes such as aada and tetA(48). Transposase was the mobile genetic element (MGE) that deserves the most attention, which maintained a relatively high abundance in each sample. Antibiotic efflux-transposase had the highest abundance of all ARG resistance mechanism-MGE types. At the end of composting, antibiotic efflux-transposase abundance in group Se_H decreased by 7.14 %, 21.96 %, and 54.92 %, respectively, compared with groups CK, Se_L, and Se_M. High-concentration nano-Se inhibited the activity of transposase to reduce the risk of horizontal transmission of antibiotic efflux ARGs. Proteobacteria and Actinobacteria were the main ARG host microorganisms, and Actinobacteria had a lower tolerance threshold for nano-Se. Network analysis and Mantel testing demonstrated that high-concentration nano-Se could weaken the association between host microorganisms and the ARG subtypes. Structural equation models showed that high-concentration nano-Se regulated the transfer of ARGs primarily by stimulating signal transduction in host microorganisms. Thus, the addition of proper concentrations of nano-Se during aerobic composting of cow manure was effective in reducing ARG exposure risk by regulating transposase and host microorganisms.