Synthesis of fluorinated leucines, valines and alanines for use in protein NMR
Ansis Maļeckis, Elwy H. Abdelkader, Iresha D. Herath, Gottfried Otting
Abstract
19 F-NMR spectra of the protein GB1 produced by cell-free protein synthesis with fluorinated amino acids substituting their canonical parent amino acids show exceptionally large chemical shift dispersions, which indicate structural conservation.
Topics & Concepts
ChemistryFluorine-19 NMRValineIsotopologueNMR spectra databaseAmino acidReagentNuclear magnetic resonance spectroscopyStereochemistryOrganic chemistryMoleculeBiochemistrySpectral lineAstronomyPhysicsFluorine in Organic ChemistryMass Spectrometry Techniques and ApplicationsHemoglobin structure and function