Litcius/Paper detail

False-Negative Results of Real-Time Reverse-Transcriptase Polymerase Chain Reaction for Severe Acute Respiratory Syndrome Coronavirus 2: Role of Deep-Learning-Based CT Diagnosis and Insights from Two Cases

Dasheng Li, Dawei Wang, Jianping Dong, Nana Wang, He Huang, Xu Haiwang, Xia Chen

2020Korean Journal of Radiology397 citationsDOIOpen Access PDF

Abstract

The epidemic of 2019 novel coronavirus, later named as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is still gradually spreading worldwide. The nucleic acid test or genetic sequencing serves as the gold standard method for confirmation of infection, yet several recent studies have reported false-negative results of real-time reverse-transcriptase polymerase chain reaction (rRT-PCR). Here, we report two representative false-negative cases and discuss the supplementary role of clinical data with rRT-PCR, including laboratory examination results and computed tomography features. Coinfection with SARS-COV-2 and other viruses has been discussed as well.

Topics & Concepts

MedicineReverse transcriptaseGold standard (test)Polymerase chain reactionCoinfectionVirologySevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)CoronavirusFalse Negative ReactionsCoronavirus disease 2019 (COVID-19)Nucleic acid testMiddle East respiratory syndrome coronavirusReverse transcription polymerase chain reactionReal-time polymerase chain reactionVirusPathologyGeneInternal medicineBiologyGeneticsMessenger RNAInfectious disease (medical specialty)DiseaseSARS-CoV-2 detection and testingCOVID-19 diagnosis using AISARS-CoV-2 and COVID-19 Research
False-Negative Results of Real-Time Reverse-Transcriptase Polymerase Chain Reaction for Severe Acute Respiratory Syndrome Coronavirus 2: Role of Deep-Learning-Based CT Diagnosis and Insights from Two Cases | Litcius