A general method for quantifying ligand binding to unmodified receptors using Gaussia luciferase
András Dávid Tóth, Dániel Garger, Susanne Prokop, Eszter Soltész‐Katona, Péter Várnai, András Balla, Gábor Turu, László Hunyady
Abstract
adrenergic receptor ligands. Our data demonstrate that GLuc-based biosensors provide a simple, sensitive, and cost-efficient platform for drug characterization and development.
Topics & Concepts
Förster resonance energy transferLigand (biochemistry)Ligand binding assayReceptorBioluminescenceChemistryBiophysicsLuciferaseBiosensorBiochemistryBiologyFluorescencePhysicsTransfectionQuantum mechanicsGenebioluminescence and chemiluminescence researchNicotinic Acetylcholine Receptors StudyMolecular Communication and Nanonetworks