Investigation of sugar binding kinetics of the E. coli sugar/H+ symporter XylE using solid-supported membrane-based electrophysiology
Andre Bazzone, Laura Tesmer, Derya Kurt, H. Ronald Kaback, Klaus Fendler, M. Gregor Madej
Abstract
for different substrates), we propose a multiple-step mechanism and postulate an energy profile for sugar translocation. We also suggest a mechanism by which d-glucose can act as an inhibitor for XylE.
Topics & Concepts
Lactose permeaseSymporterSugarMajor facilitator superfamilyChemistryBiochemistryPermeaseGlucose transporterTransporterBiophysicsKineticsChromosomal translocationBiologyEndocrinologyPhysicsQuantum mechanicsInsulinGeneBacterial Genetics and BiotechnologyProtein Structure and DynamicsRNA and protein synthesis mechanisms