Bioconversion of Phytosterols to 9-Hydroxy-3-Oxo-4,17-Pregadiene-20-Carboxylic Acid Methyl Ester by Enoyl-CoA Deficiency and Modifying Multiple Genes in Mycolicibacterium neoaurum
Chenyang Yuan, Shikui Song, Jianxin He, Jingxian Zhang, Xiangcen Liu, Eliana L. Pena, Junsong Sun, Jiping Shi, Zhengding Su, Baoguo Zhang
Abstract
C22 steroids are valuable precursors for steroid drug synthesis, but the development of C22 steroids remains unsatisfactory. This study presented a strategy for the one-step bioconversion of phytosterols to a C22 steroid drug precursor, 9-hydroxy-3-oxo-4,17-pregadiene-20-carboxylic acid methyl ester (9-OH-PDCE), by 3-ketosteroid-Δ 1 -dehydrogenase and enoyl-CoA hydratase deficiency with overexpression of 17β-hydroxysteroid dehydrogenase acyl-CoA dehydrogenase in Mycolicibacterium .
Topics & Concepts
BioconversionDehydrogenaseSteroidChemistryBiochemistryKetosteroidEnzymeFermentationIsomeraseHormoneSteroid Chemistry and BiochemistryPharmacogenetics and Drug MetabolismHormonal Regulation and Hypertension