Structural Differences in Translation Initiation between Pathogenic Trypanosomatids and Their Mammalian Hosts
Anthony Bochler, Jailson Brito Querido, Terezie Prilepskaja, Heddy Soufari, Angelita Simonetti, Mayara Lúcia Del Cistia, Lauriane Kühn, A. R. Ribeiro, Leoš Shivaya Valášek, Yaser Hashem
Abstract
, and the association of a kinetoplastid-specific DDX60-like helicase. It also reveals the 40S-binding site of the eIF5 C-terminal domain and structures of key terminal tails of several conserved eIFs underlying their activities within the PIC. Our results are corroborated by glutathione S-transferase (GST) pull-down assays in both human and T. cruzi and mass spectrometry data.
Topics & Concepts
Eukaryotic Small Ribosomal SubunitBiologyEukaryotic initiation factorEukaryotic translationEIF4ETranslation (biology)GeneticsCell biologyInitiation factorRibosomal RNAFive prime untranslated regionRNA Helicase AProtein subunitHelicaseComputational biologyMessenger RNARNAGeneRNA modifications and cancerRNA and protein synthesis mechanismsTrypanosoma species research and implications