Wastewater-based protocols for SARS-CoV-2: insights into virus concentration, extraction, and quantitation methods from two years of public health surveillance
Dagmara S. Antkiewicz, Kayley H. Janssen, Adélaïde Roguet, Hannah E. Pilch, Rebecca B. Fahney, Paige A. Mullen, Griffin N. Knuth, Devin G. Everett, Evelyn M. Doolittle, Kaitlyn King, Carter Wood, Angellica Stanley, Jocelyn D.C. Hemming, Martin M. Shafer
Abstract
A systematic analysis of widely used concentration, extraction and quantification methods, to identify which ones perform best for wastewater surveillance of SARS-CoV-2, PMMoV and BCoV. Accuracy, sensitivity, and throughput metrics were evaluated.
Topics & Concepts
WastewaterSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Coronavirus disease 2019 (COVID-19)Extraction (chemistry)2019-20 coronavirus outbreakVirologyChromatographyComputer scienceEnvironmental scienceMedicineChemistryEnvironmental engineeringInfectious disease (medical specialty)Internal medicineDiseaseOutbreakSARS-CoV-2 detection and testingBiosensors and Analytical DetectionAdvanced biosensing and bioanalysis techniques