Phenolic compounds of <i>Eugenia involucrata</i> (Myrtaceae) extracts and associated antioxidant and inhibitory effects on acetylcholinesterase and α-glucosidase
Andreza Cipriani, Adrieli Luz de Sousa, Adrielli Tenfen, Diogo Alexandre Siebert, André Luís de Gasper, Luciano Vitali, Gustavo Amadeu Micke, Michele Debiasi Alberton
Abstract
Eugenia involucrata DC. (Myrtaceae), popularly known as “cereja-do-Rio-Grande”, is a native tree from Brazil, popularly used as a hypoglycemiant. Crude hydroalcoholic extract (CHE) and fractions (insoluble (FI), dichloromethane (FDM), ethyl acetate (FEA) and butanol (FBu)) of leaves were assessed to determine the phenolic chemical composition by HPLC-ESI-MS/MS. 10 compounds were identified, being 7 new for this species: rutin, isoquercitrin, luteolin-7-O-rutinoside, mandelic acid, naringenin, luteolin-7-O-glucoside and salicylic acid. Extract and fractions showed inhibitory activity on acetylcholinesterase (AchE) enzyme (best result: IC50 = 44.19 µg mL−1, for FEA) and α-glucosidase (α-Glu) (best result: IC50 = 31.25 ± 0.15 µg mL−1, for CHE). The observed antioxidant and inhibitory activity on the AchE and α-Glu is due to, at least in part, the presence of phenolic compounds in the samples.