Combating oxidative stress and inflammation in gentamicin-induced nephrotoxicity using hydrogen-rich water
Mustafa Oguz Cumaoğlu, Mustafa Makav, Serpil DAĞ, Ayfer Yildiz Uysal, Lale Başer, Tyler W. LeBaron, Duried Alwazeer
Abstract
Gentamicin-induced nephrotoxicity primarily results from renal inflammatory cascades and increased oxidative stress. This study aims to examine the effects of hydrogen-rich water (HRW) on gentamicin-induced renal damage in rats. Thirty-two rats were equally divided into four groups, including control (no treatment), hydrogen, gentamicin, and gentamicin+hydrogen. At the end of one week, all animals were euthanized following ethical rules, and blood and tissue samples were analyzed for examining Malondialdehyde (MDA), glutathione (GSH), Tumor Necrosis Factor-Alfa (TNF-α), Tumor Necrosis Factor-Beta (TNF-β), Interleukin 6 (IL-6), endoglin, endocan, urea, creatinine, Na + , and K + parameters. Levels of 8-Hydroxyguanosine (8-OHdG), MDA, and Bax were immunohistochemically analyzed. Data showed that while MDA (control P <0.0001, H 2 P <0.0001 , Genta+H 2 P <0.0007), TNF-α (control P <0.0002, H 2 P <0.0040, Genta+H 2 P <0.0381), IL-6 (control P <0.0044 , H 2 P< 0.0070 , Genta+H 2 P <0.0109), endocan (control P <0.0460 , H 2 P <0.0286, Genta+H 2 P <0.0452), and endoglin (control P <0.0131 , H 2 P <0.0164, Genta+H 2 P <0.0397), urea (control P <0.0024 , H 2 P <0.0001 , Genta+H 2 P <0.0180), and creatinine parameters (control P <0.0017 , H 2 P <0.0178, Genta+H 2 P <0.0011 ) increased in the gentamicin group compared to the other groups , a decrease in these parameters was observed in the gentamicin+hydrogen group compared to the gentamicin group . The Genta group had greater levels of TNF-β than the control ( P <0.0042) and H2 groups ( P <0.0268). GSH content was higher in the hydrogen group compared to the gentamicin group. Immunohistochemically, 8-OHdG, MDA, and Bax expressions increased in the gentamicin group compared to the control group, whereas they decreased in the gentamicin+hydrogen group compared to the gentamicin group. Hydrogen may be an alternative treatment for oxidative stress-induced nephrotoxicity. • Hydrogen effect on gentamicin-induced nephrotoxicity in rats was studied • MDA, TNF-α, TNF-β, IL-6, endoglin, endocan, urea, and creatinine decreased in the gentamicin+hydrogen group • GSH content was higher in the hydrogen group compared to the gentamicin group • 8-OHdG, MDA, and Bax expressions decreased in the gentamicin+hydrogen group • Hydrogen may be suggested for treating gentamicin-induced renal toxicity