Statistical optimization of hydrazone-crosslinked hyaluronic acid hydrogels for protein delivery
Esther A T Mozipo, Alycia N. Galindo, Jenna D. Khachatourian, Conor G. Harris, Jonathan Dorogin, Veronica Spaulding, Madeleine R. Ford, Malvika Singhal, Kaitlin C. Fogg, Marian H. Hettiaratchi
Abstract
as an optimal hydrogel that met all of our design criteria, including displaying a gelation time of 3.7 minutes, compressive modulus of 62.1 Pa, and minimal mass change over 28 days. For protein delivery, we conjugated affinity proteins called affibodies that were specific to the osteogenic protein bone morphogenetic protein-2 (BMP-2) to HA hydrogels and demonstrated that our platform could control the release of BMP-2 over 28 days. Ultimately, our approach demonstrates the utility of DoE for optimizing hydrazone-crosslinked HA hydrogels for protein delivery.