A Scalable Suspension Platform for Generating High-Density Cultures of Universal Red Blood Cells from Human Induced Pluripotent Stem Cells
Jaichandran Sivalingam, Yu SuE, Zhong Ri Lim, Alan Tin‐Lun Lam, Alison Lee, Hsueh Lee Lim, Hong Yu Chen, Hong Kee Tan, Tushar Warrier, Jing Wen Hang, Nazmi Bin Nazir, Andy Hee‐Meng Tan, Laurent Rénia, Yuin‐Han Loh, Shaul Reuveny, Benoît Malleret, Steve Oh
Abstract
cells/mL. Functional (oxygen binding, hemoglobin characterization, membrane integrity, and fluctuations) and transcriptomics evaluations showed minimal differences between hiPSC-derived and adult-derived RBCs. The scalable agitation suspension culture differentiation process we describe here could find applications in future large-scale production of RBCs in controlled bioreactors.
Topics & Concepts
BiologyInduced pluripotent stem cellHuman Induced Pluripotent Stem CellsSuspension cultureStem cellCell biologySuspension (topology)Cell cultureMolecular biologyGeneticsEmbryonic stem cellGeneHomotopyPure mathematicsMathematics3D Printing in Biomedical ResearchPluripotent Stem Cells ResearchErythrocyte Function and Pathophysiology