SERS and Fluorescence-Active Multimodal Tessellated Scaffolds for Three-Dimensional Bioimaging
Elisa Lenzi, Dorleta Jiménez de Aberasturi, Malou Henriksen‐Lacey, Paula Piñeiro, Ayşe J. Muñiz, Joerg Lahann, Luis M. Liz‐Marzán
Abstract
-glycolic acid) (PLGA) as the base material, in which fluorescent labels and SERS-active gold nanoparticles (AuNPs) can be embedded. The combination of imaging techniques allows optimization of SERS imaging parameters for cell monitoring. The scaffolds provide anchoring points for cell adhesion, so that cell growth can be observed in a suspended 3D matrix, with multiple reference points for confocal fluorescence and SERS imaging. By prelabeling cells with SERS-encoded AuNPs and fluorophores, cell proliferation and migration can be simultaneously monitored through confocal Raman and fluorescence microscopy. These scaffolds provide a simple method to follow cell dynamics in 4D, with minimal disturbance to the tissue model.