Microarray-guided evaluation of the frequency, B-cell origins, and selectivity of human glycan-binding antibodies reveals new insights and novel antibodies
J. Sebastian Temme, Jennifer A. Crainic, Laura M. Walker, Weizhun Yang, Zibin Tan, Xuefei Huang, Jeffrey C. Gildersleeve
Abstract
memory B cells may be a promising source of such antibodies. Furthermore, some of the newly identified antibodies bound glycans for which there are no reported monoclonal antibodies available, and these may be useful as research tools, diagnostics, or therapeutic agents. Overall, the results provide insight into the types and properties of antiglycan antibodies produced by the human immune system and a framework for the identification of novel antiglycan antibodies in the future.
Topics & Concepts
AntibodyMonoclonal antibodyGlycanImmune systemBiologyMemory B cellMicroarrayB cellImmunologyMolecular biologyComputational biologyGlycoproteinGeneBiochemistryGene expressionGlycosylation and Glycoproteins ResearchMonoclonal and Polyclonal Antibodies Research