Beyond the known cuts: trypsin specificity in native proteins
João M. Gaspar, Bohdana Sokolova, Amir Ata Saei, José C. Marques, Roman A. Zubarev
Abstract
Above-filter digestion proteomics (AFDIP) was applied to quantify trypsin cleavage preferences in native HeLa cell lysates. Lysine sites were cleaved faster than arginine ones, with cleavage rates modulated by the peptide's size and isoelectric point. These trends, absent in denatured proteomes, highlight trypsin's context-dependent behavior and inform protein engineering for optimal digestibility.
Topics & Concepts
TrypsinCleavage (geology)LysineHeLaArginineChemistryPeptideBiochemistryProteomicsCellBiologyEnzymeAmino acidPaleontologyGeneFracture (geology)Advanced Proteomics Techniques and ApplicationsProtein Structure and DynamicsMachine Learning in Bioinformatics