Physicochemical property changes of <i>Dendrobium officinale</i> leaf polysaccharide <scp>LDOP‐A</scp> and it promotes <scp>GLP</scp>‐1 secretion in <scp>NCI‐H716</scp> cells by simulated saliva‐gastrointestinal digestion

Abstract

leaves by membrane separation, cellulose column, and dextran gel column. The Smith degradable products, methylation products, and nuclear magnetic resonance analysis showed that LDOP-A may be composed of →4)-Glc-(1→, →3,6)-Man-(1→, and →6)-Glc-(1→sugar residues. In vitro, simulated digestion assays showed that LDOP-A could be partially digested in the stomach and small intestine, and produced a large amount of acetic acid and butyric acid during colonic fermentation. Further cell experiment results illustrated that LDOP-A-I (LDOP-A digested by gastrointestinal tract) could induce glucagon-like peptide-1 (GLP-1) secretion in NCI-H716 cells without showing any cytotoxicity.

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