Primary culture of tracheal aspirate-derived human airway basal stem cells
Gaurang M. Amonkar, Richard Wagner, K. Bankoti, Jessica E. Shui, Xingbin Ai, Paul H. Lerou
Abstract
Patient-specific airway basal stem cells (BSCs) can be derived from tracheal aspirate (TA) samples from intubated patients, thus providing an invaluable lung stem cell derivation method that bypasses the need for lung tissue. The primary culture of BSCs provides the ideal model to study the function and differentiation of the conducting lung epithelium. This protocol outlines the specific steps for isolation, culture maintenance, passaging, freezing, thawing, differentiation, and immunofluorescence characterization of human TA-derived airway BSCs. For complete details on the use and execution of this protocol, please refer to Lu et al. (2021).
Topics & Concepts
Stem cellImmunofluorescenceAirwayLungEpitheliumRespiratory epitheliumBasal (medicine)Cell cultureBiologyPathologyCell biologyImmunologyMedicineInternal medicineSurgeryAntibodyEndocrinologyGeneticsInsulinNeonatal Respiratory Health ResearchTracheal and airway disordersTissue Engineering and Regenerative Medicine