Targeted synthesis of a trimethoxyphenyltetrahydropyrimidine analogue designed as a DNA intercalator: <i>in silico</i> , multi-spectroscopic, thermodynamic, and <i>in vitro</i> approaches
Ahmed A. Al‐Karmalawy, Ayman Abo Elmaaty, Galal Magdy, Aya Saad Radwan, Radwan Alnajjar, Moataz A. Shaldam, Arwa Omar Al Khatib, Salem Salman Almujri, Abdullah Yahya Abdullah Alzahrani, Haytham O. Tawfik
Abstract
with calf thymus DNA (ctDNA) was examined using various techniques, such as spectrofluorimetry, UV-vis spectrophotometry, viscosity measurements, ionic strength measurements, and thermodynamics to confirm its mechanism of action. Investigating the intermolecular binding interaction between small compounds and DNA can provide valuable insights for designing drugs with enhanced effectiveness and improved targeted activities.
Topics & Concepts
In silicoIntercalation (chemistry)In vitroChemistryRational designCombinatorial chemistryDNADoxorubicinStereochemistryComputational biologyBiochemistryNanotechnologyBiologyMaterials scienceOrganic chemistryGeneticsChemotherapyGeneDNA and Nucleic Acid ChemistryCancer therapeutics and mechanismsSynthesis and Biological Evaluation