A visual CRISPR/dCas9-mediated enzyme-linked immunosorbent assay for nucleic acid detection with single-base specificity
Shanshan Zhai, Yao Yang, Yuhua Wu, Jun Li, Yunjing Li, Gang Wu, Jingang Liang, Hongfei Gao
Topics & Concepts
Nucleic acidChemistryCRISPRRecombinase Polymerase AmplificationCas9DNANucleaseSubgenomic mRNAGuide RNAComputational biologyMolecular biologyTrans-activating crRNAMolecular beaconBiochemistryBiologyGeneLoop-mediated isothermal amplificationOligonucleotideCRISPR and Genetic EngineeringAdvanced biosensing and bioanalysis techniquesBiosensors and Analytical Detection