Defence of Brassicaceae plants against generalist and specialised insect pests through the development of myrosinase mutants: A review
Mubasher Ahmad Malik, Jorge Poveda, Diana L. Zuluaga, Luigi Boccaccio, Zeshan Hassan, Muhammad Usman Akram, Jamin Ali
Abstract
The flowering plant family Brassicaceae includes several species of agricultural and scientific significance, such as Arabidopsis and Brassica . Brassica crops face biotic stress from a variety of insect pests, including sucking and chewing pests. Under normal conditions, glucosinolates are stable compounds within plant cells. However, plant tissue is disrupted glucosinolates and their associated enzyme myrosinase, which are stored in separate cellular compartments, interact. Upon insect attack, myrosinase catalyses the converstion of glucosinolate into toxic compoud including isothiocyanates, thiocyanates and nitriles. Recently, research efforts worldwide have fouced on developing myrosinase mutants within Brassicaceae species, which enhance defence against insect pest by incresaing the concentration of glucosinolate hydrolysis products and producing a pungent odour. The strategy for myrosinase mutation and the resulting plant defence mechanisms have been extensively studied over the past three decades. Despite the diverse techniques employed to generate myrosinase including Breeding, RNA Interferance (RNAi), Ablation and CRISPR-Ca12a technology, these plant exhibit both synergistic and antagonistic effect on the growth and performance of various chewing and sucking insect pests of Brassica crops. In conclusion new genomic technologies may allow for precise modifications to myrosinase genes in Brassica , enhancing resistance to insect pests. Understanding insect adaptations and integrating genetically modified crops with traditional pest management can create a holistic approach to reduce pesticide reliance. Furthermore, exploring secondary metabolites and conducting extensive field trials will be essential for evaluating the effectiveness and ecological impact of these genetic improvements. This review aims to provide in-depth knowledge of the myrosinase mutants developed and the different strategies adopted to mutate the myrosinase gene in Brassicaceae.