Glycoside-specific metabolomics combined with precursor isotopic labeling for characterizing plant glycosyltransferases
Jie Wu, Wentao Zhu, Xiaotong Shan, Jinyue Liu, Lingling Zhao, Qiao Zhao
Abstract
]-phenylalanine precursor. Our method revealed that UGT72Es could use coumarins as substrates, and genetic evidence showed that UGT72Es endowed plants with enhanced tolerance to low iron availability under alkaline conditions. Using the newly developed method, the function of UGT78D2 was also evaluated. These case studies suggest that this method can substantially contribute to the characterization of UGTs and efficiently investigate glycosylation processes, the complexity of which have been highly underestimated.
Topics & Concepts
GlycosylationGlycosideMetabolomicsGlycosyltransferaseMonolignolBiochemistryGlycomeBiologyArabidopsisMutantChemistryBiosynthesisBotanyEnzymeGeneGlycanBioinformaticsGlycoproteinPlant Gene Expression AnalysisPlant biochemistry and biosynthesisNatural product bioactivities and synthesis