Alpha‐amylase immobilized on polycaprolactone‐grafted magnetic nanoparticles: improving stability and reusability
Mahshid Defaei, Asghar Taheri‐Kafrani, Mehran Miroliaei, Parichehreh Yaghmaei
Abstract
Abstract BACKGROUND Widespread application of enzymes for research and industrial purposes is dependent on enhancing the catalytic capacity. One promising approach to this end is the immobilization of enzymes on novel nanomaterials. In this study, a novel nanocarrier was developed for the immobilization of enzyme on polycaprolactone (PCL)‐functionalized magnetic nanoparticles. RESULT Structural and physicochemical characterization of the PCL‐modified magnetic nanocarrier was achieved via Fourier‐transform infrared (FTIR) spectra, scanning electron microscopy (SEM), transmission electron microscopy (TEM), thermogravimetric analysis (TGA) and vibration sample magnetometry (VSM). In order to determine the optimal conditions for immobilization, different parameters such as pH, temperature, reaction time and enzyme concentration were evaluated. Based on the results, the optimal biocatalytic activity of immobilized α‐amylase was obtained at 65 °C and pH 6.5. Under appropriate conditions, the immobilized α‐amylase showed improved storage stability in comparison with free one. Additionally, the reusability of the immobilized system demonstrated >50% enzyme activity, at optimal pH and temperature, after five reaction cycles. CONCLUSION This finding could be useful in the application of immobilized enzymes for analytical purposes, industrial exploitations, biotechnology, biomedical engineering and other bio‐applications. © 2020 Society of Chemical Industry