Oligosaccharide analysis of the backbone structure of the characteristic polysaccharide of Dendrobium officinale
Tin-Long Wong, Lifeng Li, Jixia Zhang, Quanwei Zhang, Xueting Zhang, Lishuang Zhou, Hau-Yee Fung, Lei Feng, Huiyuan Cheng, Chuying Huo, Man Liu, Wan-Rong Bao, Wenjie Wu, Cheuk-Hei Lai, Su‐Ping Bai, Shaoping Nie, Pema‐Tenzin Puno, Clara Bik‐San Lau, Ping-Chung Leung, Quan‐Bin Han, Han-Dong Sun
Abstract
The backbone of Dendrobium officinale polysaccharide (DOP) was reported to be glucomannan. But the dispute continues due to the limitation of conventional analysis methods (e.g., the overlapped NMR signals). Herein, we describe an oligosaccharide sequencing analysis that successfully corrects the backbone to mannan. This analysis involves producing oligosaccharide fragments from mild acid hydrolysis of DOP followed by p-aminobenzoic ethyl ester (ABEE) derivatization. Then, a series of oligosaccharides with varied polymerization degrees (DP = 2–10) were isolated and identified by LC-MS profiling, monosaccharide composition analysis, linkage analysis, and 1D-/2D-NMR elucidation. Finally, we conclude that the backbone of DOP is a β-D-1,4-mannan which is confirmed by other analysis such as comparison with reference mannan. This oligosaccharide analysis is more straightforward and reliable by transforming the complicated polymers into oligomers which have more accurate and convincing MS and NMR data.