An alternative σ factor σ<sup>L</sup><sub>sl</sub> regulates lincomycin production in <i>Streptomyces lincolnensis</i>
Bingbing Tu, Yue Mao, Ruida Wang, Yajing Kang, Ye Jiang, Huizhan Zhang, Haizhen Wu
Abstract
Abstract Lincomycin produced by Streptomyces lincolnensis is a critical antibacterial antibiotic in the clinical. To further understand the regulatory mechanism of lincomycin biosynthesis, we identified an alternative σ factor, σ L sl , in Streptomyces lincolnensis NRRL 2936. Deletion of sigL sl resulted in an increase in cell growth but a decrease in lincomycin production. σ L sl boosted lincomycin biosynthesis by directly stimulating the transcription of four genes ( lmbD, lmbV, lmrC , and lmbU ) within the lincomycin biosynthetic lmb gene cluster. Besides, σ L sl participated in lincomycin biosynthesis by directly stimulating the transcription of mshC , a gene responsible for MSH synthesis. In conclusion, our findings demonstrated that σ L sl plays a direct regulatory role in lincomycin biosynthesis. This study extends the understanding of molecular mechanisms of lincomycin biosynthetic regulation.