Screening <i>PAX9</i>, <i>MSX1</i> and <i>WNT10A</i> Mutations in 4 Iranian Families with Non-Syndromic Tooth Agenesis.
Shiva Safari, Asghar Ebadifar, Hossien Najmabadi, Koorosh Kamali, Seyedeh Sedigheh Abedini
Abstract
BACKGROUND: are commonly reported in the literature. Hence, the aim of this study was to investigate the mutations of these genes in 4 Iranian families with non-syndromic tooth agenesis. METHODS: DNA extractions from peripheral blood cells of patients with non-syndromic tooth agenesis from 4 unrelated Iranian families were performed by salting out method, and the candidate genes were amplified then followed by Sanger sequencing method. RESULTS: gene. CONCLUSION: may not play a role in non-syndromic tooth agenesis among Iranian cases.
Topics & Concepts
Sanger sequencingAXIN2Missense mutationGeneticsBiologyGeneSingle-nucleotide polymorphismCandidate geneMutationGenotypeWnt signaling pathwaydental development and anomaliesOral and Maxillofacial PathologyCleft Lip and Palate Research