The Chemistry and Biology of Bactobolin: A 10-Year Collaboration with Natural Product Chemist Extraordinaire Jon Clardy
E. Peter Greenberg, Josephine R. Chandler, Mohammad R. Seyedsayamdost
Abstract
was reported as part of a collaboration between the Greenberg and Clardy laboratories in 2010. The collaboration sparked a series of studies leading to the discovery of new analogues and associated structure-activity relationships, the identification of the bactobolin biosynthetic gene cluster and assembly of its unusual amino acid building block, the molecular target of and resistance to the antibiotic, and finally an X-ray crystal structure of the ribosome-bactobolin complex. Herein, we review the collaborations that led to our current understanding of the chemistry and biology of bactobolin.
Topics & Concepts
Natural productChemistryGene clusterComputational biologyStereochemistryBiologyBiochemistryGeneMicrobial Natural Products and BiosynthesisRNA and protein synthesis mechanismsPlant biochemistry and biosynthesis