Genome Capture Sequencing Selectively Enriches Bacterial DNA and Enables Genome-Wide Measurement of Intrastrain Genetic Diversity in Human Infections
Hillary S. Hayden, Snehal Joshi, Matthew C. Radey, Anh T. Vo, Cara M Forsberg, Sarah J. Morgan, Adam Waalkes, Elizabeth A. Holmes, Sara M. Klee, Mary J. Emond, Pradeep K. Singh, Stephen J. Salipante
Abstract
Genetic diversity evolves in bacterial strains during human infections and could affect disease manifestations and treatment resistance. However, the extent of diversity present in vivo and its changes over time are difficult to measure by conventional methods.
Topics & Concepts
GenomeHuman genomeDNABacterial genome sizeGeneticsBiologyComputational biologyDNA sequencingGeneAntibiotic Resistance in BacteriaBacterial Genetics and BiotechnologyBacteriophages and microbial interactions