High Bioaffinity Controllable Assembly Nanocarrier UiO-66-NH<sub>2</sub>@Quantum Dot-Based Immunochromatographic Assay for Simultaneous Detection of Five Mycotoxins in Cereals and Feed
Jiayi Chen, Zehao Yang, Jianpeng Zhang, Xing Shen, Zhenlin Xu, Xiangmei Li, Hongtao Lei
Abstract
Herein, the UiO-66-NH 2 @quantum dot (NU66@QD) was synthesized with excellent fluorescence intensity and biocompatibility, which was used to develop a multiple immunochromatographic assay (ICA) for the detection of aflatoxin B 1 (AFB 1 ), fumonisin B 1 (FB 1 ), deoxynivalenol (DON), T-2 toxins (T-2), and zearalenone (ZEN) in cereals and feed. Five monoclonal antibodies and NU66@QD were efficiently labeled by a one-step mixed method to form a multiple detection probe. The limits of detection of the proposed NU66@QD-ICA for AFB 1 /FB 1 /DON/T-2/ZEN were 0.04/0.28/0.25/0.09/0.08 μg/kg. The recoveries ranged from 82.83–117.44%, with the coefficient of variation from 2.88–11.80%. A parallel analysis in 35 naturally contaminated cereal and feed samples was confirmed by LC-MS/MS, and the results showed a good correlation ( R 2 > 0.9), indicating the practical reliability of the multiple NU66@QD-ICA. Overall, the introduction of the novel nanomaterial NU66@QD provides a highly sensitive and efficient multiplex detection strategy for the development of ICA.