Ultrasensitive and Specific Identification of Monkeypox Virus Congo Basin and West African Strains Using a CRISPR/Cas12b-Based Platform
Xu Chen, Wei Yuan, Xinggui Yang, Yuanfang Shi, Xiaoyan Zeng, Junfei Huang, Yi Wang, Shijun Li
Abstract
Monkeypox (MPX), a reemerging zoonotic disease caused by monkeypox virus (MPXV), causes a smallpox-like disease in humans. Early diagnosis is critical to prevent MPX epidemics. Here, CRISPR/Cas12b was integrated with LAMP amplification to devise a novel CRISPR-MPXV approach to achieve highly specific, ultrasensitive, rapid, and visual identification of MPXV Congo Basin and West African strains.
Topics & Concepts
MonkeypoxIdentification (biology)VirologyCRISPRBiologyGeographyComputational biologyGeneticsEcologyGeneVacciniaRecombinant DNAPoxvirus research and outbreaksCRISPR and Genetic EngineeringPlant Virus Research Studies